Rapid Identification of Methicillin-Resistant Staphylococcus aureus Transmission in Hospitals by Use of Phage-Derived Open Reading Frame Typing Enhanced by Multiplex PCR and Reverse Line Blot Assay
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چکیده
منابع مشابه
Identification of the classical enterotoxin genes of Staphylococcus aureus in various foods by multiplex PCR assay
Background: An annual update of information about the prevalence of Staphylococcus aureus and staphylococcal enterotoxins (SEs) genes is required in every geographic area. Aims: This study was conducted to investigate the prevalence of the bacterium and type of associated enterotoxin genes in different food samples, using multiplex polymerase ...
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Surveillance is very important to prevent the nosocomial spread of methicillin-resistant Staphylococcus aureus (MRSA), and infection sources and routes have historically been identified using molecular and epidemiological genotyping with pulsed-field gel electrophoresis. However, phage-open reading frame typing (POT) has recently been developed. Here, we investigated whether POT would be useful...
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We previously published a simple multiplex PCR assay for quick-screening of Staphylococcal Cassette Chromosome mec (SCCmec) types I-V, which has been extensively used worldwide. An updated assay is described here and the changes serve to make this rapid assay more accurate and reliable in facilitating identification of most common and major SCCmec types.
متن کاملidentification o f clinical methicillin and mupirocin-resistant staphylococcus aureus by multiplex-pcr
background : infections resulted from multi-resistant staphylococcus aureus are increasing worldwide. in the present study, a multiplex-pcr assay for the detection of antibiotic resistance genes among s. aureus clinical isolates and for the concomitant identification of these isolates was described. methods : a total of 127 s. aureus isolates were collected from clinical specimens at three teac...
متن کاملSimultaneous identification of 14 genital microorganisms in urine by use of a multiplex PCR-based reverse line blot assay.
The aim of this study was to develop and evaluate a sensitive method for the simultaneous identification of 14 urogenital potential pathogens. A multiplex PCR-based reverse line blot (mPCR/RLB) assay was developed to detect 14 urogenital pathogens or putative pathogens, namely Trichomonas vaginalis, Streptococcus pneumoniae, Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma parvum, U. ur...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2010
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.02201-09